| Abstract Detail
Systematics Section/ASPT Gostel, Morgan [1], Weeks, Andrea [2]. Developing novel EPIC primers for shallow-scale phylogenetic inference and assessing their phylogenetic utility in a recently derived lineage of Madagascan Commiphora (Burseraceae). This paper describes the development of four novel exon-primed, intron-crossing (EPIC) markers from putative conserved, nuclear intron regions for phylogenetic inference among closely related species of Commiphora as well as across the Burseraceae. We have evaluated their phylogenetic utility by sampling closely related species in a recent radiation of the myrrh genus, Commiphora, in Madagascar and also more broadly among several genera in the Burseraceae. Commiphora is the most species-rich genus in the frankincense and myrrh family (Burseraceae) and in Madagascar the genus has experienced multiple, rapid radiations throughout the western dry deciduous forest and southern spiny bush vegetation zones. Elucidating interspecific relationships among species of Madagascan Commiphora (ca. 50 spp.) will allow us to test several competing hypotheses regarding the historical biogeography of the genus in Madagascar. Despite tremendous ecological and morphological diversity among species of Commiphora, current molecular analysis has been unable to completely resolve interspecific relationships within two large clades within Madagascar. Although the markers sampled for species of Commiphora are, to date, among the most common and informative for infrageneric phylogenetic analyses in other angiosperm lineages (including ITS, ETS, psbA-trnH, ndhF-rpl32, trnD-trnT), a lack of variation among these sequences for these regions in Commiphora has failed to provide sufficient resolution to interpret patterns of diversification, particularly among species belonging the two diverse Malagasy clades. The identification of informative, low-copy number markers could help resolve phylogenetic relationships among closely related species in Commiphora and more broadly in the Burseraceae. Broader Impacts:
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1 - George Mason University, Environmental Science And Policy, David King Hall, MSN 5F2, 4400 University Dr., Fairfax, VA, 22030-4444, USA
2 - George Mason University, 4400 University Drive MSN 5F2, Fairfax, VA, 22030, USA
Keywords:
Burseraceae
phylogeny
nuclear markers
marker development
Madagascar.
Presentation Type: Oral Paper:Papers for Sections
Session: 19
Location: Napoleon Ballroom/Riverside Hilton
Date: Tuesday, July 30th, 2013
Time: 8:45 AM
Number: 19002
Abstract ID:667
Candidate for Awards:None |
