Abstract Detail

Systematics Section/ASPT

Peery, Rhiannon [1], Mathews, Sarah [2], Downie, Stephen [3].

Update on the quest to resolve higher-level relationships in Apiaceae subfamily Apioideae.

Apiaceae is a large family of flowering plants, with over 400 genera and 4,000 species. Its largest subfamily, Apioideae, comprises some 3,000 species, and includes many economically important members, such as Daucus and Pastinaca, as well as highly toxic plants, like Conium maculatum. Apioideae comprises 28 monophyletic tribes and other major clades that have yet to be named. Fourteen of these clades, such as tribes Apieae, Careae, and Selineae, comprise the apioid superclade, a morphologically heterogeneous group of uncertain relationships. Resolution of evolutionary relationships within the apioid superclade is the last major problem of Apiaceae systematics, but limited insights have been gained from work to date because the molecular markers used previously are too conserved or too rapidly evolving to discern tribal– and lower–level taxonomic relationships. To aid in resolving these relationships, this study adds sequence data from three plastid loci heretofore not used in Apiaceae systematic study: 5,155 characters from psbM to psbD, 400 characters from trnH to psbA, and 775 characters from rps19 to rpl2. Furthermore, three newly discovered rare genomic changes within the plastid genome were included as additional characters. These changes include a 200-1000 bp insertion at the large single copy – inverted repeat boundary near trnH, a 2,178 bp inversion of trnH and psbA causing trnH to be adjacent to trnK, and a 571 bp inversion of the region between psbM to trnT. Combining these new structural data with previously published DNA matrices incorporating sequences from nrDNA ITS and several other plastid loci produced a final matrix of over 12,000 characters for 179 species sampled from all 14 tribes and major clades. Phylogenetic analyses of this data set increased resolution from a comb phylogeny to a phylogeny having 20 well-supported nodes. Unfortunately most of the resolution is near the tips and not in the backbone of the phylogeny. Additional relationships were delineated, but support values were lower. While these rare genomic changes and sequence data added strong support for some nodes, the addition of nuclear data is needed, not only to bolster the phylogenetic hypothesis but to test for possible instances of prior hybridization. Currently we are exploring the utility of the nuclear gene phyA for further resolution of phylogenetic relationships within the apioid superclade.

Broader Impacts:

1 - University of Illinois At Urbana-Champaign, 265 Morrill Hall, 505 S Goodwin Ave, Urbana, IL, 61801-3707, USA
2 - Harvard University, Harvard University Herbaria, 22 Divinity Avenue, Cambridge, MA, 02138, USA
3 - University of Illinois, Department of Plant Biology, 265 MORRILL HALL, 505 S GOODWIN AVE, Urbana, IL, 61801, USA

Keywords:
Apiaceae
phyA
Systematics.

Presentation Type: Oral Paper:Papers for Sections
Session: 33
Location: Rosedown/Riverside Hilton
Date: Tuesday, July 30th, 2013
Time: 3:15 PM
Number: 33007
Abstract ID:922
Candidate for Awards:None