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Abstract Detail

Recent Topics Posters

Riley, Rachel [1], Chester, Michael [1], Soltis, Pamela [2], Soltis, Douglas [3].

Do chromosomal-level changes account for homeolog loss in the neoallotetraploid T. mirus (Asteraceae)?

The recently formed allotetraploid Tragopogon mirus provides an opportunity to examine genomic changes approximately 40 generations after its formation. One of the consequences of allopolyploidy is ‘homeolog loss’ where a gene from one of the diploid parents is lost in the derived allopolyploid. Previous investigations of recently formed allopolyploid Tragopogon species have identified homeolog loss, but so far the genetic/genomic basis for this loss remains unclear. To test whether large-scale chromosomal changes, such as aneuploidy and/or non-reciprocal translocations are responsible, we examined homeolog loss for four genes in 37 karyotyped individuals of T. mirus. For each plant we used PCR, cleaved amplified polymorphic sequence analysis (CAPS), and genomic in situ hybridization (GISH). We observed no uniparental loss for T. porrifolius homeologs, whereas the T. dubius homeolog was lost for all four genes in two individuals. An inspection of the karyotypes from these two individuals did not reveal chromosomal changes that could account for the loss of genetic material of T. dubius. Therefore we conclude that the apparent losses of T. dubius genes are not caused through large-scale chromosomal alterations but possibly much smaller scale events such as deletions, rearrangements or gene conversion events.

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1 - University of Florida, Department of Biology, Gainesville, FL, 32611, USA
2 - University of Florida, Florida Museum of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA
3 - University of Florida, Biology and Florida Museum of Natural History, Gainesville, FL, 32611, USA


Presentation Type: Recent Topics Poster
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PRT009
Abstract ID:1295
Candidate for Awards:None

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