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Abstract Detail

Recent Topics Posters

McCann, Tyler [1], Latvis, Maribeth [2], Soltis, Douglas [3], Soltis, Pamela [4].

Phylogenetic Relationships of Agalinis kingsii and Conservation Implications.

Agalinis kingsii is a rare Caribbean endemic found growing along the northeastern coast of Grand Cayman in a single sedge wetland. The current conservation program lacks phylogenetic data for A. kingsii and the relationship with its close relatives. Phylogenetic knowledge of organisms is critical to effective conservation programs that correctly identify and protect independent evolutionary lineages. Pettengill and Neel (2011) identified barcoding gene regions successful in distinguishing closely related species using North American Agalinis as the test system (rpoB, rps2, psbA-trnH, trnL-trnF, ITS). DNA from these gene regions was amplified, allowing us to add specimens of A. kingsii to gene matrices with up to 45 total Agalinis species sampled across the genus. We also included expanded sampling of Agalinis maritima, with the goal of elucidating the relationship of A. kingsii to this closely related and taxonomically confused species. These sequences were compared using a maximum likelihood method in RAxML with 1000 bootstrap replicates, under various partitioning schemes. The clade containing accessions of A. maritima and A. kingsii was supported with a bootstrap value of 100% in all topologies. Resolution within the clade was low, and bootstrap support was <50%. It therefore remains unclear whether A. kingsii represents a distinct lineage from A. maritima, or if it is nested within this recognized species. Future studies utilizing microsatellites and/or new nuclear gene or spacer regions may help further elucidate the taxonomic status of Agalinis kingsii and inform local conservation efforts.

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1 - University of Florida, Florida Museum of Natural History, Gainesville, FL
2 - Florida Museum of Natural History, Florida Museum of Natural History, Dickinson Hall, Museum Rd., Gainesville, FL, 32611, USA
3 - University of Florida, Biology and Florida Museum of Natural History, Gainesville, FL, 32605, USA
4 - University of Florida, Florida Museum of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA

DNA barcodes

Presentation Type: Recent Topics Poster
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PRT045
Abstract ID:1342
Candidate for Awards:None

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