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Abstract Detail

Systematics Section/ASPT

Moraski, Ryan [1], Stull, Gregory [2], Soltis, Douglas [3], Soltis, Pamela [4].

Next-generation plastome sequencing of Guzmania (Bromeliaceae): Implications for a robust species-level bromeliad phylogeny.

The bromeliad family (Bromeliaceae), with more than 3300 species assigned to 58 genera, is a morphologically diverse and ecologically important group of flowering plants. The family is thought to have undergone a recent, rapid adaptive radiation beginning around 20 million years, diversifying across most of the Neotropics. Much progress has been made in resolving the major phylogenetic backbone of the family through the analysis of several coding and non-coding plastid DNA markers. However, attempts to elucidate inter- and intrageneric relationships have been confounded by a combination of a low rate of bromeliad plastid DNA evolution and the young age of extant lineages. Guzmania (subf. Tillandsioideae) is one of the most species-rich groups of bromeliads, with over 210 species from across most of the range of Bromeliaceae. Here we present the results of a plastome-targeted enrichment and low-cost next-generation sequencing approach to the sequencing of complete plastid genomes for three species of Guzmania using the Illumina GAIIx sequencing platform. We report statistics on plastome coverage and assembly and discuss the importance of a multiplex genomic DNA sequencing approach for estimating the phylogeny of a rapid, recently radiated group of plants. The application of this approach should significantly improve our understanding of the intrageneric variation in plastid DNA sequences within the Bromeliaceae.

Broader Impacts:

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1 - Florida Museum of Natural History, University of Florida, Department of Biology, PO Box 117800, Gainesville, FL, 32611-7800, USA
2 - University of Florida, Florida Museum of Natural HIstory, Dickinson Hall, Museum Rd. And Newel Dr., Gainesville, N/A, 32611, USA
3 - University of Florida, Biology/FLMNH, Dickinson Hall , MUseum Road, Gainesville, FL, 32611-7800, USA
4 - University of Florida, Florida Museum of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA

Illumina GAIIx

Presentation Type: Poster:Posters for Sections
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PSY011
Abstract ID:288
Candidate for Awards:None

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