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Abstract Detail

Genomics / Proteomics

Lappin, Fiona [1], Medert, Charles [1], Mardonovich, Sandra [1], Wu, Meng [1], Moore, Richard [1].

Redefining the Pseudoautosomal region boundaries of Carica papaya X chromosome.

The sex chromosomes of Carica papaya are characterized by a relatively small region of suppressed recombination the X region and the MSY (Male Specific Y region) flanked by two larger, recombining pseudoautosomal regions (PARs). PARs are essential in the proper segregation of the sex chromosomes during meiosis and persist in the sex chromosomes of most species. Our investigation aimed to redefine the border of one of the C. papaya PARs by analyzing the patterns of polymorphism at eight loci previously identified as X-linked, but that have no identified Y-linked homologues. These loci span an ~1Mbp region adjacent to the MSY/X homologous region and were resequenced in six male individuals. Two of these eight loci exhibit a clear molecular signature of X/Y-linkage; each male contains two divergent alleles on par with the divergence levels exhibited in identified X/Y linked loci. The other six loci have autosomal patterns of polymorphism; alleles are highly similar, some males contain only a single allele, and there is evidence of recombinant alleles. By mapping these along the X chromosome we were able to identify the region of ~95kb where boundary between the PAR and X lies, reducing the previous estimated size of the X by ~ 2Mbp. A clearly defined PAR border will allow for further investigation into the evolution of expansion of the PAR, especially when studied in a subject that has relatively young sex chromosomes like that of papaya.

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1 - Miami University, Department of Botany, 316 E.High St, Oxford, OH, 45056, USA

none specified

Presentation Type: Poster:Posters for Topics
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PGP001
Abstract ID:298
Candidate for Awards:None

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