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Abstract Detail


Leonard, Opal [1], Murrin, William [2], Morris, Ashley [3].

Characterization of novel nuclear and chloroplast microsatellite loci in New World Illicium.

Illicium comprises 30-40 species distributed in the southeastern United States, Mexico, and southeastern Asia. The genus is divided into New and Old World clades based on molecular and morphological data. The New World clade is a relatively small group of seven species, exhibiting a disjunct distribution across the southeastern United States, Mexico, and the Greater Antilles. The goal of this study is to develop robust markers to study the evolutionary history, population structure, and clonal structure of New World Illicium. Novel nuclear microsatellite primers were designed by a commercial facility using Illumina-based sequencing of genomic DNA enriched for microsatellite loci; 55 are currently being tested for utility in New World Illicium. Ten previously published universal chloroplast microsatellite loci are also being tested for use in this group. Eight accessions representing six of the seven New World species are being used for testing: I. floridanum, I. mexicanum, I. parviflorum, I. hottense, I. guajaibonense, and I. cubense. Loci were initially PCR amplified then screened for successful amplification using gel electrophoresis. Loci were chosen for subsequent optimization based on whether or not they amplified, variability across accessions, and clarity of observed patterns. Subsequent reactions were fluorescently labeled and run on an ABI 3730 XL DNA analyzer. Data were analyzed using GeneMarker MPT software and checked manually by eye. Of the 55 nuclear loci initially ordered, 25 have been tested. Of those, 14 were selected for additional optimization. Of the 10 chloroplast microsatellite loci ordered, five were selected for additional optimization. Preliminary results from chloroplast loci are consistent with previous systematic findings. We ultimately plan to use these microsatellite markers to test specific biogeographic hypotheses and address long-standing taxonomic issues in this group.

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1 - Middle Tennessee State University, Department of Biology, PO Box 60, 1301 E Main St, Murfreesboro, TN, 37132, USA
2 -
3 - Middle Tennessee State University, Department of Biology, PO Box 60, 1301 E. Main Street, Murfreesboro, TN, 37132, USA


Presentation Type: Poster:Posters for Topics
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PBG006
Abstract ID:355
Candidate for Awards:None

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