| Abstract Detail
Evolutionary Developmental Biology (Evo-Devo) Liu, Xiang [1], Zhang, Jian [1], Abuahmad, Ahmad [1], Franks, Robert [2], Xie, Deyu [1], Xiang, Qiuyun (Jenny) [3]. Cornus TFL1-like genes extended vegetative growth and rescued indeterminate inflorescence in Arabidopsis. The TERMINAL FLOWER 1 (TFL1) is known to be one of key genes regulating inflorescence development in Arabidopsis by determining meristem shoot identity. However, the roles of TFL1 homologs in development of inflorescences in most other plants have remained unknown. In this report, we show functions of two TFL1 homologs from Cornus species. Open reading frame fragments of CocaTFL1 and CoflTFL1 were amplified from cDNA libraries of flowers of Cornus canadensis and Cornus florida (Cornaceae) and then subcloned into binary vectors under the control of 35S promoter. These two cDNAs were then transformed into wild type col-0 (WT) and strong mutant tfl1-18 of Arabidopsis via the floral-dip method of Agrobacterium-mediated transformation, respectively. Phenotypes of transgenic plants were analyzed in progenies of T1 and T2, and homologous lines. The overexpression of CocaTFL1 and CoflTFL1 in WT plants led to extension of vegetative growth and increase of rosette leaves, cauline leaves, and branches. Meanwhile, the overexpression of these two cDNAs alone in tfl1-18 background rescued the phenotype of the indeterminate inflorescence. In comparison, the rescuing extent of the CoflTFL1 overexpression was stronger than that of CocaTFL1.Our data suggest that CocaTFL1 and CoflTFL1 have similar function to that ofTFL1 in Arabidopsis and were able to regulate flower transition, which is also supported by low sequence variation of the genes between these taxa. This finding suggests potential differences in function of TFL1 orthologs from different species of Cornus. Broader Impacts:
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Related Links: http://www4.ncsu.edu/~qyxiang/
1 - North Carolina State University, Plant Biology, Raleigh, NC, 27695, USA 2 - North Carolina State University, Genetics, Raleigh, NC, 27695, USA 3 - North Carolina State University, Gardner Hall 2115, CAMPUS BOX 7612, RALEIGH, NC, 27695-7612, USA
Keywords: Cornus TFL1-like genes Inflorescence development transformation Arabidopsis.
Presentation Type: Oral Paper:Papers for Topics Session: 42 Location: Jasperwood/Riverside Hilton Date: Wednesday, July 31st, 2013 Time: 11:30 AM Number: 42006 Abstract ID:376 Candidate for Awards:None |