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Abstract Detail

Economic Botany Section

Moscoe, Lauren [1], Emshwiller, Eve [2].

Comparison between SSR and AFLP analyses of oca (Oxalis tuberosa: Oxalidaceae).

Identification of clones is an important objective in genetic diversity studies of vegetatively propagated crops. Such studies should employ molecular markers that efficiently and consistently detect both variation and similarity among germplasm accessions. The recent development of microsatellites (simple sequence repeats; SSRs) for the Andean tuber crop oca (Oxalis tuberosa Molina) offers potential advantages (e.g., single locus markers, co-dominance) over the previously available methods of amplified fragment length polymorphism (AFLP) and inter-simple sequence repeats (ISSR). We generated SSR data for a collection of 28 oca samples previously analyzed with AFLP and compared fineness of differentiation and reliability of clone identification between molecular marker systems. SSR data support hypotheses of morphotype-based clustering as revealed by AFLP analyses, but many samples that appear genetically identical with respect to SSR loci are similar, but not identical, with respect to AFLPs. This discrepancy in clone identification may be attributed to factors such as non-specificity of AFLPs or to lower mutation rates in the utilized SSR loci. Increased sampling and replications will further contribute to ongoing discussions about the roles and relationships among different molecular markers in investigations of clonally propagated crops.

Broader Impacts:

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1 - University of Wisconsin-Madison, Botany, 301 Birge Hall, 430 Lincoln Drive, Madison, Wisconsin, 53706, United States
2 - University of Wisconsin-Madison, Botany Dept, 321 Birge Hall, 430 Lincoln Drive, Madison, WI, 53706, USA

clonally propagated crops
Oxalis tuberosa

Presentation Type: Poster:Posters for Sections
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PEN008
Abstract ID:446
Candidate for Awards:Economic Botany Section best poster

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