Abstract Detail

Systematics Section/ASPT

Kim, Hye Min [1], Oh, Sang-Hun [2], Kim, Chan-Soo [3], Bhandari, Gauri Shankar [1], Park, Chong-Wook [1].

DNA barcoding of the Korean Orchidaceae.

With about 20,000 species, Orchidaceae is the one of the largest and most diverse families of the flowering plants, and contains many economically important species used as cultivated ornamentals, medicines, and for extracting fragrance materials. In Korea, the family is represented by 42 genera and ca. 88 species, many of which are considered as rare and endangered species in the country. Among the 64 taxa of the endangered species of the vascular plants listed by the Korean government, 10 species belong to Orchidaceae, indicating that the critical status of Korean orchids in conservation biology. Accurate and rapid identification of the orchid material, even in the sterile condition, is one of the fundamental steps in conservation of the biodiversity of Korean orchids and in utilization of natural products of orchids. We developed a framework for DNA barcode database of Korean Orchidaceae, as part of the biodiversity study of the family, which also included generation of interactive key based on morphological characters. Five chloroplast DNA regions, including rbcL, matK, atpF-atpH, psbK-psbI, and trnH-psbA, were evaluated for 122 accessions of 82 species in 32 genera. The five candidate DNA barcode regions were successfully amplified with a set of universal primers in all of the samples examined. Analysis of resolution power for species based on neighbor-joining trees, reconstructed from the sequence data separately and in combination, showed that employment of combined regions increased the power of resolution compared with utilization of the single region. The combination of the rbcL, matK, atpF-atpH, and trnH-psbA regions provided the best performance to discriminate all of the orchid species evaluated.

Broader Impacts:

1 - Seoul National University, School of Biological Sciences, Gwanak 599 Gwanak-ro, Gwanak-gu, Seoul, 151-747, Republic of Korea
2 - Daejeon University, 7417 Basic Science Building, 63 College Avenue, Daejeon, N/A, 300-716, Korea
3 - Korea Forest Research Institute, Warm-temperste and subtropical forest research center , 22 Donneko-gil, Seogwipo, Jeju, 697-050, Republic of Korea

Keywords:
Orchidaceae
DNA barcoding
Chloroplast DNA
rbcL
matK
atpF-atpH
psbK-psbI
trnH-psbA.

Presentation Type: Poster:Posters for Sections
Session: P
Location: Grand Salon A - D/Riverside Hilton
Date: Monday, July 29th, 2013
Time: 5:30 PM
Number: PSY061
Abstract ID:483
Candidate for Awards:None