Abstract Detail

Systematics Section/ASPT

Godden, Grant [1], Cortez, Maria Beatriz [2], Soltis, Douglas [3], Soltis, Pamela [4].

Out of the Bushes and Into the Trees: Next-generation Sequencing Approaches for Resolving the Phylogeny of Lamiaceae.

Recent breakthroughs and technological advancements in molecular laboratory techniques, next-generation sequencing (NGS), and computational biology are arming systematic biologists with a new arsenal of powerful approaches and tools for phylogenetic applications. This arsenal provides an economical and efficient means for acquiring large datasets from both nuclear and organellar genomes that may help resolve many difficult phylogenetic problems, especially those for which 5-10 molecular markers fail to adequately resolve phylogeny; these methods also afford an opportunity to explore use of alternative phylogenetic approaches (e.g., gene tree reconciliation) that facilitate the reconstruction of relationships despite possible discordances among individual gene histories. We selected Hedeoma and related New World genera in subtribe Menthinae (Lamiaceae)—a taxonomically problematic and difficult to resolve mint group—as a model system in which to test the efficacy of several new approaches for large-scale data acquisition on NGS platforms, marker development and targeted sequencing, and phylogenetics that may prove effective for resolving relationships across the remainder of the family. We present the first phylogenomic results for Lamiaceae, inferred from a maximum likelihood analysis of 96 nearly complete chloroplast genomes representing 53 accessions from Hedeoma and 43 taxa from 16 genera of New World Menthinae (Acanthomintha, Blephilia, Clinopodium, Conradina, Cunila, Dicerandra, Glechon, Hesperozygis, Kurzamra, Mentha, Monarda, Monardella, Poliomintha, Pycnanthemum, Rhabdocaulon, Rhododon, and Thymus), and discuss their implications for future phylogenetic studies of mints.

Broader Impacts:

1 - University of Florida, Department of Biology and Florida Museum of Natural History, 301 Dickinson Hall, Gainesville, FL, 32611-7800, USA
2 - Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz SN, Barão Geraldo, Campinas, São Paulo, 13083-970, Brazil
3 - University of Florida, Biology and Florida Museum of Natural History, Gainesville, FL, 32605, USA
4 - University of Florida, Florida Museum of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA

Keywords:
Chloroplast capture
Hedeoma
Illumina
Lamiaceae
Menthinae
Phylogenomics
Next-generation sequencing
massively parallel sequencing
phylogeny
chloroplast genome.

Presentation Type: Oral Paper:Papers for Sections
Session: 49
Location: Marlborough A/Riverside Hilton
Date: Wednesday, July 31st, 2013
Time: 3:00 PM
Number: 49007
Abstract ID:910
Candidate for Awards:None