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Abstract Detail



Genetics Section

Jordon-Thaden, Ingrid [1], Viccini, Lyderson [2], Buggs, Richard [3], Chester, Michael [4], Cruz Da Silva, Ana Veruska [5], Chamala, Srikar [6], Davenport, Ruth [6], Wu, Wei [7], Schnable, Patrick S. [7], Barbazuk, W. Brad [6], Soltis, Douglas [8], Soltis, Pamela [9].

Differential gene expression and loss in two natural and synthetic allotetraploid Tragopogon species (Asteraceae) and their diploid progenitors.

We present an overview of our large study of differential gene loss and expression in two recently formed natural allotetraploid species (Tragopogon miscellus and T. mirus), their diploid parents (T. pratensis, T. dubius, and T. porrifolius), and three generations of synthetic allotetraploids (T. miscellus and T. mirus). We established a randomized block experiment for comparison of diploids and natural and synthetic allotetraploids. Using SNPs identified from 454 and Illumina sequencing of the diploids, we designed ~7000 SNP possible primers for Sequenom Mass-ARRAY analysis of gDNA and cDNA from 392 individuals, which were all grown simultaneously under controlled conditions. One month after germination, two leaves where harvested from the same individuals on the same day, in hopes to eliminate any expression variability from environmental or daily fluctuations. Overall, ~500 SNPs for each pair of parents and their respective allotetraploid derivative were used (~1000 total). SNP variation was used to assess homeolog loss and changes in gene expression in the allotetraploids relative to their parents and to compare broadly sampled wild individuals with synthetics. Approximately 20% of the individuals tested show evidence of homeolog loss, with slightly more loss detected in the natural allotetraploids compared to the synthetic tetraploids. Changes in gene expression will also be discussed.

Broader Impacts:


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1 - University of California Berkeley, University & Jepson Herbaria, 1001 Valley Life Sciences Bldg, Berkeley, CA, 94720, USA
2 - Universidade Federal de Juiz de Fora, Biology, Laboratory of Genetics, Juiz de Fora, MG, 36036-900, Brazil
3 - Queen Mary, University of London, The School of Biological and Chemical Sciences, Mile End Road, London, E1 4NS, UK
4 - University of Florida, Department of Biology, Gainesville, FL, 32605, USA
5 - Embrapa Coastal Tablelands, Aracaju, SE, 49025, Brazil
6 - University of Florida, Genetics Institute, Gainesville, FL, 32610, USA
7 - Iowa State University, Center for Plant Genomics, Ames, IA, 50011, USA
8 - University of Florida, Biology and Florida Museum of Natural History, Gainesville, FL, 32605, USA
9 - University of Florida, Florida Museum of Natural History, PO BOX 117800, Gainesville, FL, 32611-7800, USA

Keywords:
allotetraploid
Asteraceae
Polyploidy
Gene expression
SNP
Tragopogon.

Presentation Type: Oral Paper:Papers for Sections
Session: 21
Location: Marlborough B/Riverside Hilton
Date: Tuesday, July 30th, 2013
Time: 9:00 AM
Number: 21002
Abstract ID:936
Candidate for Awards:Margaret Menzel Award


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